pCAG-ERT2CreERT2
(Plasmid #13777)

• Purpose: Mammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen)
• Depositing Lab: Connie Cepko
• Publication: Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1027-32. doi: 10.1073/pnas.0610155104

Backbone

• Vector backbone: pCAGEN
• Backbone size w/o insert (bp): 4779
• Vector type: Mammalian Expression, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ERT2-Cre-ERT2 fusion protein
• Alt name: inducible Cre
• Species: Bacteriophage P1
• Insert Size (bp): 2951

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pCAG-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ERT2CreERT2 was modified from pCreERT2 (Feil et al. BBRC, 237, 752-757 (1997) obtained from Dr. P. Chambon (Universite Louis Pasteur).
• Articles Citing this Plasmid:
  • 47 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

4-hydroxitamoxifen-responsible Cre.
Kozak consensus sequence was added before the start ATG.
The insert can be cut out with EcoRI (SmaI) and NotI.