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Addgene

CALCOCO1 W47A-mycDDK
(Plasmid #137769)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 137769 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV6-Entry
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CALCOCO1 W47A
  • Species
    H. sapiens (human)
  • Mutation
    W47A
  • Promoter CMV
  • Tag / Fusion Protein
    • mycDDK (C terminal on insert)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CALCOCO1 W47A-mycDDK was a gift from Carol Mercer (Addgene plasmid # 137769 ; http://n2t.net/addgene:137769 ; RRID:Addgene_137769)

  • For your References section:

    Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy. Stefely JA, Zhang Y, Freiberger EC, Kwiecien NW, Thomas HE, Davis AM, Lowry ND, Vincent CE, Shishkova E, Clark NA, Medvedovic M, Coon JJ, Pagliarini DJ, Mercer CA. Autophagy. 2020 Jan 23. doi: 10.1080/15548627.2020.1719746. 10.1080/15548627.2020.1719746 PubMed 31971854
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