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Addgene

pAF302
(Plasmid #137752)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 137752 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRPF185
  • Backbone manufacturer
    Fagan and Fairweather
  • Backbone size w/o insert (bp) 7200
  • Total vector size (bp) 7566
  • Vector type
    Bacterial Expression
  • Selectable markers
    thiamphenicol

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hupA-linker-hibitopt
  • Species
    Clostridioides difficile
  • Insert Size (bp)
    366
  • GenBank ID
    YP_001090017.1
  • Promoter Ptet
  • Tag / Fusion Protein
    • HiBiTopt (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CACCTCCTTTTTGACTTTAAGCCTACGAATACC (NF793)
  • 3′ sequencing primer CACCGACGAGCAAGGCAAGACCG (NF794)
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAF302 was a gift from Wiep Klaas Smits (Addgene plasmid # 137752 ; http://n2t.net/addgene:137752 ; RRID:Addgene_137752)

  • For your References section:

    The C-terminal domain of Clostridioides difficile TcdC is exposed on the bacterial cell surface. Oliveira Paiva AM, de Jong L, Friggen AH, Smits WK, Corver J. J Bacteriol. 2020 Aug 31. pii: JB.00771-19. doi: 10.1128/JB.00771-19. 10.1128/JB.00771-19 PubMed 32868401
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