-
PurposepCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable marker
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 137735 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneColE1/AMP
- Backbone size w/o insert (bp) 5600
- Total vector size (bp) 9000
-
Vector typeBacterial Expression, Yeast Expression
-
Selectable markersTRP1
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Top10
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameyeTadA1.0-T7 RNAP
-
SpeciesS. cerevisiae (budding yeast)
-
Insert Size (bp)3200
-
MutationE366G (See depositor comment below)
Resource Information
-
Supplemental Documents
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Depositor confirms E366G does not affect plasmid function.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCS4333 yeTadA1.0-T7 RNAP was a gift from Christina Smolke (Addgene plasmid # 137735 ; http://n2t.net/addgene:137735 ; RRID:Addgene_137735) -
For your References section:
Polymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering. Cravens A, Jamil OK, Kong D, Sockolosky JT, Smolke CD. Nat Commun. 2021 Mar 11;12(1):1579. doi: 10.1038/s41467-021-21876-z. 10.1038/s41467-021-21876-z PubMed 33707425
