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pLV-PGK-DIO-mCherry-nega miR
(Plasmid #137732)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 137732 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV-PGK-GFP
  • Backbone manufacturer
    Åkerblom et al., 2013
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    control microRNA
  • gRNA/shRNA sequence
    TGC TGA AAT GTA CTG CGC GTG GAG ACG TTT TGG CCA CTG ACT GAC GTC TCC ACG CAG TAC ATT T
  • Species
    M. musculus (mouse)
  • Tag / Fusion Protein
    • mChherry (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer tgacaacgggccacaactcc
  • 3′ sequencing primer CCCTCGTTGACCGAATCACC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Thermo Fisher Scientific

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

the website for the BLOCK-iT RNAi Designer

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-PGK-DIO-mCherry-nega miR was a gift from Tomoyuki Furuyashiki (Addgene plasmid # 137732 ; http://n2t.net/addgene:137732 ; RRID:Addgene_137732)

  • For your References section:

    The Innate Immune Receptors TLR2/4 Mediate Repeated Social Defeat Stress-Induced Social Avoidance through Prefrontal Microglial Activation. Nie X, Kitaoka S, Tanaka K, Segi-Nishida E, Imoto Y, Ogawa A, Nakano F, Tomohiro A, Nakayama K, Taniguchi M, Mimori-Kiyosue Y, Kakizuka A, Narumiya S, Furuyashiki T. Neuron. 2018 Aug 8;99(3):464-479.e7. doi: 10.1016/j.neuron.2018.06.035. Epub 2018 Jul 19. 10.1016/j.neuron.2018.06.035 PubMed 30033154
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