Pprm1-luciferase; his-3
(Plasmid #137728)

• Purpose: Communication-activated luciferase in Neurospora crassa
• Depositing Lab: Louise Glass
• Publication: Fischer et al Genetics. 2019 Apr;211(4):1255-1267. doi: 10.1534/genetics.118.301780. Epub 2019 Feb 4.

Backbone

• Vector backbone: pMF272
• Vector type: Luciferase ; Neurospora Crassa Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Luciferase
• Alt name: Luc
• Species: Firefly
• Insert Size (bp): 1741
• Mutation: Codon-optimized for N. crassa

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: Unknown

Gene/Insert 2

• Gene/Insert name: Pprm-1 Promoter
• Species: Neurospora crassa
• Insert Size (bp): 1757

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • Pprm1-Luciferase_his3.gb
• A portion of this plasmid was derived from a plasmid made by: Firefly luciferase codon-optimized for Neurospora crassa was received from Jay Dunlap's Lab (Gooch et al, 2007, Eukaryotic Cell)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry