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Addgene

pGL2084
(Plasmid #137726)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 137726 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRPA-ISL
  • Vector type
    RNAi ; Trypanosoma brucei tet inducible stem loop
  • Selectable markers
    Hygromycin ; Hyg resistance is only generated by correct recombinantion into T.b.brucei 2T1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    Plasmid has tendency to recombine due to the inverted repeats and the toxic ccdB genes. if grown at temperatures above 25c. MUST be grown slowly at room temperature.
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pGL2084 is a Gateway adapted version of the original pRPA-ISL vector developed by Alsford and Horn, 2008.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL2084 was a gift from Jeremy Mottram (Addgene plasmid # 137726 ; http://n2t.net/addgene:137726 ; RRID:Addgene_137726)

  • For your References section:

    Regulators of Trypanosoma brucei cell cycle progression and differentiation identified using a kinome-wide RNAi screen. Jones NG, Thomas EB, Brown E, Dickens NJ, Hammarton TC, Mottram JC. PLoS Pathog. 2014 Jan;10(1):e1003886. doi: 10.1371/journal.ppat.1003886. Epub 2014 Jan 16. 10.1371/journal.ppat.1003886 PubMed 24453978
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