pTE3987
(Plasmid #137716)

• Purpose: Expresses dLbCas12a-RVRR-BE in mammalian cells.
• Depositing Lab: Ervin Welker
• Publication: Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 5763098. doi: 10.1093/nar/gkaa110.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 3399
• Total vector size (bp): 8166
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dLbCas12a-RVRR-BE
• Alt name: inactive Lachnospiraceae bacterium Cas12a nuclease RVRR variant in fusion with Apobec base editor
• Insert Size (bp): 4767
• Mutation: G532A, K538V, Y542R, K595R
• Promoter: CMV
• Tags / Fusion Proteins:
  • SV40 NLS (N terminal on insert)
  • APOBEC-1 (N terminal on insert)
  • SV40 NLS (C terminal on insert)
  • UGI (C terminal on insert)
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CGCAAATGGGCGGTAGGCGTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry