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Addgene

pVE13300
(Plasmid #137715)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 137715 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5402
  • Total vector size (bp) 9272
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    impLbCas12a
  • Alt name
    human codon optimized enhanced LbCas12a
  • Insert Size (bp)
    3870
  • Promoter CMV
  • Tags / Fusion Proteins
    • SV40 NLS (N terminal on insert)
    • NLS (C terminal on insert)
    • 3xHA (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVE13300 was a gift from Ervin Welker (Addgene plasmid # 137715 ; http://n2t.net/addgene:137715 ; RRID:Addgene_137715)

  • For your References section:

    Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases. Toth E, Varga E, Kulcsar PI, Kocsis-Jutka V, Krausz SL, Nyeste A, Welker Z, Huszar K, Ligeti Z, Talas A, Welker E. Nucleic Acids Res. 2020 Feb 28. pii: 5763098. doi: 10.1093/nar/gkaa110. 10.1093/nar/gkaa110 PubMed 32107556
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