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Addgene

gRNA telo/GFP
(Plasmid #137713)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 137713 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV
  • Vector type
    Mammalian Expression, AAV, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA telo + GFP
  • Promoter U6/RSV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Addgene NGS finds some deletions and discrepancies at the ITRs within the plasmid compared to the depositor's sequence. These differences are not known to affect plasmid function, as the depositor was able to package the ITR-flanked transgene efficiently into AAV particles despite the noted sequence discrepancies.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    gRNA telo/GFP was a gift from Dominik Niopek (Addgene plasmid # 137713 ; http://n2t.net/addgene:137713 ; RRID:Addgene_137713)
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