dCas9-3×RFP-P2A-wtAcrIIA4/gRNA telo
(Plasmid
#137712)
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PurposeVector co-expressing dCas9-3×RFP-P2A-wtAcrIIA4 and sgRNA telo
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 137712 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneLentiviral vector
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Vector typeMammalian Expression, Lentiviral, CRISPR, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedCas9-3×RFP-P2A-wtAcrIIA4/gRNA telo
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SpeciesSynthetic
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-fw (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
dCas9-3×RFP-P2A-wtAcrIIA4/gRNA telo was a gift from Dominik Niopek (Addgene plasmid # 137712 ; http://n2t.net/addgene:137712 ; RRID:Addgene_137712)