pPS1494
(Plasmid
#1377)
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Depositing Lab
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 1377 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonena
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepGAL-REV-GFP
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Speciesna
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
350 bp SalI-PstI fragment containing Rev from pPS1493 in PstI-XhoI pPS904.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPS1494 was a gift from Pamela Silver (Addgene plasmid # 1377 ; http://n2t.net/addgene:1377 ; RRID:Addgene_1377) -
For your References section:
A member of the Ran-binding protein family, Yrb2p, is involved in nuclear protein export. Taura T, Krebber H, Silver PA. Proc Natl Acad Sci U S A 1998 Jun 23;95(13):7427-32. 10.1073/pnas.95.13.7427 PubMed 9636166