pHes1-DsRed
(Plasmid #13767)

• Depositing Lab: Connie Cepko
• Publication: Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1027-32. doi: 10.1073/pnas.0610155104

Backbone

• Vector backbone: pCAGEN
• Backbone size w/o insert (bp): 3087
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Hes1 promoter
• Insert Size (bp): 1828
• Tag / Fusion Protein:
  • DsRed2 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: na
• 3′ sequencing primer: DsRed1-N

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The mouse Hes1 promoter is specific for progenitor cells in the retina. This promoter is also weakly active in Muller glia in the differentiated retina.