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Addgene

pHM073Env
(Plasmid #137669)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 137669 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1D/V5-His-TOPO
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HIV-1 Envelope Glycoprotein
  • Species
    HIV-1
  • Insert Size (bp)
    2553
  • GenBank ID
    KY213719.1

Cloning Information

  • Cloning method TOPO Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HIV-1 infected Patient Samples from Singapore, reverse transcribed RNA cloned into expression vector

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHM073Env was a gift from Anjali Joshi (Addgene plasmid # 137669 ; http://n2t.net/addgene:137669 ; RRID:Addgene_137669)

  • For your References section:

    HIV-1 subtype CRF01_AE and B differ in utilization of low levels of CCR5, Maraviroc susceptibility and potential N-glycosylation sites. Joshi A, Cox EK, Sedano MJ, Punke EB, Lee RT, Maurer-Stroh S, Kaur P, Ng OT, Garg H. Virology. 2017 Dec;512:222-233. doi: 10.1016/j.virol.2017.09.026. Epub 2017 Oct 9. 10.1016/j.virol.2017.09.026 PubMed 29020646
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