pSHDY-Prha-mVenus_rhaS
(Plasmid
#137662)
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PurposeConjugative vector derived from pSHDY. Constitutive expression of the activator rhaS, as well as rhamnose-inducible expression of mVenus.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 137662 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSHDY
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Backbone manufacturerAnna Behle
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePrha:mVenus
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SpeciesE. coli
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MutationrhaS (Arg218Leu)
- Promoter Prha
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer acagtcataacaagccatg (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/757948v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSHDY-Prha-mVenus_rhaS was a gift from Ilka Axmann (Addgene plasmid # 137662 ; http://n2t.net/addgene:137662 ; RRID:Addgene_137662) -
For your References section:
Comparative Dose-Response Analysis of Inducible Promoters in Cyanobacteria. Behle A, Saake P, Germann AT, Dienst D, Axmann IM. ACS Synth Biol. 2020 Apr 17;9(4):843-855. doi: 10.1021/acssynbio.9b00505. Epub 2020 Mar 17. 10.1021/acssynbio.9b00505 PubMed 32134640
