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pNrl-DsRed
(Plasmid #13764)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13764 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGEN
  • Backbone size w/o insert (bp) 3062
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nrl promoter
  • Insert Size (bp)
    3943
  • Tag / Fusion Protein
    • DsRed2 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer na
  • 3′ sequencing primer DsRed1-N
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mouse Nrl promoter was isolated by genomic PCR using B6 mouse genomic DNA as a template.
The Nrl promoter-DsRed is specific for rod photorecetor cells in the retina.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNrl-DsRed was a gift from Connie Cepko (Addgene plasmid # 13764 ; http://n2t.net/addgene:13764 ; RRID:Addgene_13764)

  • For your References section:

    Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T, Cepko CL. Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1027-32. doi: 10.1073/pnas.0610155104 10.1073/pnas.0610155104 PubMed 17209010
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