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pcDNA3 PARL-FLAG-CT H335G
(Plasmid #13749)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13749 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Presenilin-associated rhomboid-like
  • Alt name
    PARL
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1164
  • Mutation
    Changed catalytic His 335 to Gly
  • GenBank ID
    AF197937
  • Entrez Gene
    PARL (a.k.a. PRO2207, PSARL, PSARL1, PSENIP2, RHBDS1)
  • Entrez Gene
    PARL (a.k.a. PARL)
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer GTACGGTGGGAGGTCTATAT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 PARL-FLAG-CT H335G was a gift from Luca Pellegrini (Addgene plasmid # 13749 ; http://n2t.net/addgene:13749 ; RRID:Addgene_13749)

  • For your References section:

    Mitochondrial rhomboid PARL regulates cytochrome c release during apoptosis via OPA1-dependent cristae remodeling. Cipolat S, Rudka T, Hartmann D, Costa V, Serneels L, Craessaerts K, Metzger K, Frezza C, Annaert W, D'Adamio L, Derks C, Dejaegere T, Pellegrini L, D'Hooge R, Scorrano L, De Strooper B. Cell. 2006 Jul 14. 126(1):163-75. 10.1016/j.cell.2006.06.021 PubMed 16839884
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