ACS2 Truncated
(Plasmid #13748)

• Depositing Lab: John Denu
• Publication: Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 103(27):10230-5.

Backbone

• Vector backbone: pQE80
• Backbone manufacturer: Qiagen
• Backbone size w/o insert (bp): 4700
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Truncated Acetyl CoA Sythetase 2
• Alt name: AceCS2
• Species: M. musculus (mouse)
• Insert Size (bp): 1932
• Mutation: Contains amino acids 39–682
• GenBank ID: NM_080575
• Entrez Gene: Acss1 (a.k.a. Acas2, Acas2l, AceCS2, AI788978, 1110032O15Rik)
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pQE80 sequencing vector

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry