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Addgene

pBS/U6/hTID1
(Plasmid #13745)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13745 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBS/U6
  • Backbone manufacturer
    Ambion
  • Backbone size w/o insert (bp) 3292
  • Vector type
    RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hTid1 siRNA
  • Alt name
    hTid1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    48
  • Mutation
    Inverted repeat of 21 bp with 6 nuc. spacer.
  • GenBank ID
    AF061749
  • Entrez Gene
    DNAJA3 (a.k.a. HCA57, TID1, Tid1-L, Tid1-S, hTID-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ApaI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Target sequence: GGGGTCAACAAGGAGTTCACC.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS/U6/hTID1 was a gift from Karl Munger (Addgene plasmid # 13745 ; http://n2t.net/addgene:13745 ; RRID:Addgene_13745)

  • For your References section:

    Depletion of physiological levels of the human TID1 protein renders cancer cell lines resistant to apoptosis mediated by multiple exogenous stimuli. Edwards KM, Munger K. Oncogene. 2004 Nov 4. 23(52):8419-31. 10.1038/sj.onc.1207732 PubMed 15156195
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