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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13743 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGREENLANTERN-1
- Backbone size w/o insert (bp) 4996
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Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemouse Kif3B
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SpeciesM. musculus (mouse)
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Insert Size (bp)2300
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Entrez GeneKif3b (a.k.a. AI854312, AW549267, mKIAA0359)
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Tag
/ Fusion Protein
- GFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Not1 (unknown if destroyed)
- 3′ cloning site Not1 (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL-FLKif3B was a gift from Linda Wordeman (Addgene plasmid # 13743 ; http://n2t.net/addgene:13743 ; RRID:Addgene_13743) -
For your References section:
Expression and partial characterization of kinesin-related proteins in differentiating and adult skeletal muscle. Ginkel LM, Wordeman L. Mol Biol Cell. 2000 Dec . 11(12):4143-58. 10.1091/mbc.11.12.4143 PubMed 11102514