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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13740 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepQE-80
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Backbone manufacturerQiagen
- Backbone size w/o insert (bp) 4700
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSIRT7
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SpeciesH. sapiens (human)
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Insert Size (bp)1200
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GenBank IDNM_016538
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Entrez GeneSIRT7 (a.k.a. SIR2L7)
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SphI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Derived from pcDNA3.1 SIRT1–7 with a FLAG tag, obtained from E. Verdin (University of California, San Francisco). Molecular Weight: 44898 Da.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SIRT7.4 was a gift from John Denu (Addgene plasmid # 13740 ; http://n2t.net/addgene:13740 ; RRID:Addgene_13740) -
For your References section:
Sirtuins deacetylate and activate mammalian acetyl-CoA synthetases. Hallows WC, Lee S, Denu JM. Proc Natl Acad Sci U S A. 2006 Jul 5. 103(27):10230-5. 10.1073/pnas.0604392103 PubMed 16790548