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Addgene

SIRT1.1
(Plasmid #13735)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13735 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE-80
  • Backbone manufacturer
    Qiagen
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SIRT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2010
  • GenBank ID
    NM_012238
  • Entrez Gene
    SIRT1 (a.k.a. SIR2, SIR2L1, SIR2alpha)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Truncated form of SIRT1. Missing amino acids 6-83. Molecular Weight: 81681 Da.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SIRT1.1 was a gift from John Denu (Addgene plasmid # 13735 ; http://n2t.net/addgene:13735 ; RRID:Addgene_13735)

  • For your References section:

    Sirtuins deacetylate and activate mammalian acetyl-CoA synthetases. Hallows WC, Lee S, Denu JM. Proc Natl Acad Sci U S A. 2006 Jul 5. 103(27):10230-5. 10.1073/pnas.0604392103 PubMed 16790548
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