pHsSR beta deltaN Flag
(Plasmid #13731)

• Depositing Lab: Thomas Schwartz
• Publication: Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 103(18):6823-8.

Backbone

• Vector backbone: pET28a-derived
• Backbone size w/o insert (bp): 5332
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Plasmid grows at 37, expression optimal at 30 degrees or 18 degrees
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Signal recognition particle receptor β subunit
• Alt name: SRβ
• Species: H. sapiens (human)
• Insert Size (bp): 639
• Mutation: expresses residues 59-271 of Homo sapiens SRβ with a thrombin protease cleavable N-terminal 6xHis tag snd a C-terminal Flag tag
• Entrez Gene: SRPRB (a.k.a. APMCF1)
• Tags / Fusion Proteins:
  • 6xHis (N terminal on backbone)
  • FLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde1 (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry