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Addgene

pPS2042
(Plasmid #1373)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1373 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRS314
  • Backbone size w/o insert (bp) 4785
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    U1A (1-94)
  • Alt name
    U1A
  • Species
    H. sapiens (human)
  • Mutation
    U1A (1-94)
  • Entrez Gene
    RNU1-4 (a.k.a. HSD2, HSD5, HSD6, HSD7, RNU1, RNU1D2, RNU1E2, RNU1F1, RNU1G1, RNU1G2, U1B2, U1D2, U1E2, U1F)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CEN plasmid, GAL1 promoter

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS2042 was a gift from Pamela Silver (Addgene plasmid # 1373 ; http://n2t.net/addgene:1373 ; RRID:Addgene_1373)
  • For your References section:

    Pre-mRNA processing factors are required for nuclear export. Brodsky AS, Silver PA. RNA 2000 Dec;6(12):1737-49. 10.1017/S1355838200001059 PubMed 11142374