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Addgene

pScSR beta deltaN Flag
(Plasmid #13729)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13729 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a-derived
  • Backbone size w/o insert (bp) 5332
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Plasmid grows at 37, expression optimal at 30 degrees or 18 degrees
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Signal recognition particle receptor β subunit
  • Alt name
    SRβ
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    642
  • Mutation
    expresses residues 31-244 of S. cerevisiae SRβ with a thrombin protease cleavable N-terminal 6xHis tag and a C-terminal Flag tag
  • Entrez Gene
    SRP102
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • Flag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde1 (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7 promoter
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pScSR beta deltaN Flag was a gift from Thomas Schwartz (Addgene plasmid # 13729 ; http://n2t.net/addgene:13729 ; RRID:Addgene_13729)

  • For your References section:

    Homodimerization of the G protein SRbeta in the nucleotide-free state involves proline cis/trans isomerization in the switch II region. Schwartz TU, Schmidt D, Brohawn SG, Blobel G. Proc Natl Acad Sci U S A. 2006 May 2. 103(18):6823-8. 10.1073/pnas.0602083103 PubMed 16627619
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