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pCpSRb-5
(Plasmid #13727)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13727 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a derived
  • Backbone size w/o insert (bp) 5312
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Plasmid grows at 37, expression optimal at 30 degrees or 18 degrees
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Signal recognition particle receptor β subunit
  • Alt name
    SRβ
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    591
  • Mutation
    SRβ circularly permuted D210E188 improved solubility from construct reported in paper
  • Entrez Gene
    SRP102
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde1 (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Construct is expressed with a precission protease cleavable 6xHis tag

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCpSRb-5 was a gift from Thomas Schwartz (Addgene plasmid # 13727 ; http://n2t.net/addgene:13727 ; RRID:Addgene_13727)

  • For your References section:

    Homodimerization of the G protein SRbeta in the nucleotide-free state involves proline cis/trans isomerization in the switch II region. Schwartz TU, Schmidt D, Brohawn SG, Blobel G. Proc Natl Acad Sci U S A. 2006 May 2. 103(18):6823-8. 10.1073/pnas.0602083103 PubMed 16627619
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