pCpSRb-4
(Plasmid
#13726)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13726 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a-derived
- Backbone size w/o insert (bp) 5312
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsExpression is optimal at 30 degrees
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSignal recognition particle receptor β subunit
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Alt nameSRβ
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)576
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MutationCircularly permuted SRβD210K183 with Precission protease cleavable 6x-His tag
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Entrez GeneSRP102
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Tag
/ Fusion Protein
- 6xHis (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nde1 (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCpSRb-4 was a gift from Thomas Schwartz (Addgene plasmid # 13726 ; http://n2t.net/addgene:13726 ; RRID:Addgene_13726) -
For your References section:
Homodimerization of the G protein SRbeta in the nucleotide-free state involves proline cis/trans isomerization in the switch II region. Schwartz TU, Schmidt D, Brohawn SG, Blobel G. Proc Natl Acad Sci U S A. 2006 May 2. 103(18):6823-8. 10.1073/pnas.0602083103 PubMed 16627619