Skip to main content
Addgene

PRPF4.001.169
(Plasmid #137233)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 137233 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET15-MHL
  • Backbone size w/o insert (bp) 5740
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PRPF4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    507
  • Mutation
    001-169
  • Entrez Gene
    PRPF4 (a.k.a. HPRP4, HPRP4P, PRP4, Prp4p, RP70, SNRNP60)
  • Tag / Fusion Protein
    • (His)6-TEV (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7F and LIC primer: ttgtatttccagggcATGGCTTCCTCGCGAGCCT
  • 3′ sequencing primer T7R and LIC primer: caagcttcgtcatcaCTATTCATGATACCAGGTTTGCTGATAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

insert+fusion tag if applicable MHHHHHHSSGRENLYFQGMASSRASSTQATKTKAPDDLVAPVVKKPHIYYGSLEEKERERLAKGESGILGKDGLKAGIEAGNINITSGEVFEIEEHISERQAEVLAEFERRKRARQINVSTDDSEVKACLRALGEPITLFGEGPAERRERLRNILSVVGTDALKKTKKDDEKSKKSKEEYQQTWYHE sequence of insert only (no fusion tag) MASSRASSTQATKTKAPDDLVAPVVKKPHIYYGSLEEKERERLAKGESGILGKDGLKAGIEAGNINITSGEVFEIEEHISERQAEVLAEFERRKRARQINVSTDDSEVKACLRALGEPITLFGEGPAERRERLRNILSVVGTDALKKTKKDDEKSKKSKEEYQQTWYHE

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PRPF4.001.169 was a gift from Tara Davis (Addgene plasmid # 137233 ; http://n2t.net/addgene:137233 ; RRID:Addgene_137233)

  • For your References section:

    The spliceosomal proteins PPIH and PRPF4 exhibit bi-partite binding. Rajiv C, Jackson SR, Cocklin S, Eisenmesser EZ, Davis TL. Biochem J. 2017 Oct 25;474(21):3689-3704. doi: 10.1042/BCJ20170366. 10.1042/BCJ20170366 PubMed 28935721
Related items: