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Addgene

pPS2040
(Plasmid #1372)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1372 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRS426
  • Backbone size w/o insert (bp) 5726
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PGK1-U1A-ASH1 3' UTR
  • Alt name
    PGK1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    PGK1 (a.k.a. YCR012W)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PGK1 promoter and PGK1 ORF (XhoI/BamHI fragment), U1A hairpins (BamHI/BglII fragment), ASH1 3' UTR (SpeI/NotI fragment), ADH2 terminator (NotI/SacII fragment), 2 micron plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS2040 was a gift from Pamela Silver (Addgene plasmid # 1372 ; http://n2t.net/addgene:1372 ; RRID:Addgene_1372)
  • For your References section:

    Pre-mRNA processing factors are required for nuclear export. Brodsky AS, Silver PA. RNA 2000 Dec;6(12):1737-49. 10.1017/S1355838200001059 PubMed 11142374