Skip to main content
Addgene

Cbh_v5 AAV-saCBE_KKH C-terminal
(Plasmid #137184)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 137184 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    px601
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    v5 AAV-saCBE_KKH C-terminal
  • Alt name
    JL844
  • gRNA/shRNA sequence
    BsmBI dummy protospacer
  • Species
    Synthetic
  • Mutation
    E782K;N968K;R1015H conferring recognition of NNNRRT PAMs
  • Promoter Cbh

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SaCas9 split between Lys534/Cys535; clone in protospacer by cutting with BsmBI/Esp3I and ligating annealed oligos as described in methods.. Inserts were Gibson-cloned into BshTI/BglII-restricted backbones to avoid ITR PCR.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cbh_v5 AAV-saCBE_KKH C-terminal was a gift from David Liu (Addgene plasmid # 137184 ; http://n2t.net/addgene:137184 ; RRID:Addgene_137184)

  • For your References section:

    Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Levy JM, Yeh WH, Pendse N, Davis JR, Hennessey E, Butcher R, Koblan LW, Comander J, Liu Q, Liu DR. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14. 10.1038/s41551-019-0501-5 PubMed 31937940
Related items:
Commonly requested with: