Cbh_v5 AAV-saCBE N-terminal
(Plasmid #137182)

• Purpose: AAV genome: expresses the N-terminal of S. aureus v5 AAV-CBE from the Cbh promoter, U6-sgRNA
• Depositing Lab: David Liu
• Publication: Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14.

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Backbone

• Vector backbone: px601
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: v5 AAV-saCBE N-terminal
• Alt name: JL842
• gRNA/shRNA sequence: BsmBI dummy protospacer
• Species: Synthetic
• Mutation: Cas9 D10A
• Promoter: Cbh

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • Cbh_v5 AAV-saCBE N-terminal.gb
• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

SaCas9 split between Lys534/Cys535; clone in protospacer by cutting with BsmBI/Esp3I and ligating annealed oligos as described in methods. Inserts were Gibson-cloned into BshTI/BglII-restricted backbones to avoid ITR PCR.