Cbh_v5 AAV-ABE C-terminal
(Plasmid #137178)

• Purpose: AAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNA
• Depositing Lab: David Liu
• Publication: Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14.

Backbone

• Vector backbone: px601
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: v5 AAV-CBE C-terminal; U6-protospacer
• Alt name: JL646
• gRNA/shRNA sequence: RFP dropout cassette - cut out with BsmBI
• Species: Synthetic
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • Cbh_v5 AAV-ABE C-terminal.gb
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Clone in protospacer by cutting with BsmBI/Esp3I, gel-extracting product to remove RFP dropout cassette, and ligating annealed oligos as described in methods. Background colonies will be RFP+. Inserts were Gibson-cloned into BshTI/BglII-restricted backbones to avoid ITR PCR.