pB-actin 16 Early
(Plasmid
#13711)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13711 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepB-actin
- Backbone size w/o insert (bp) 7500
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHPV 16 early region
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Alt nameHPV 16 genome
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SpeciesH. sapiens (human)
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Insert Size (bp)4400
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MutationIncl. small part of L2
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GenBank IDNC_001526
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Entrez GeneE4 (a.k.a. HpV16gp5)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Sal 1 (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pB-actin 16 Early was a gift from Karl Munger (Addgene plasmid # 13711 ; http://n2t.net/addgene:13711 ; RRID:Addgene_13711) -
For your References section:
The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes. Münger K, Phelps WC, Bubb V, Howley PM, Schlegel R. J Virol. 1989 Oct . 63(10):4417-21. PubMed 2476573