pFD-NIa-Protease-TVMV
(Plasmid
#137109)
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PurposeiFLinkC Linker cloning
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 137109 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFD
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNIa Protease TVMV
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFD-NIa-Protease-TVMV was a gift from Viktor Stein (Addgene plasmid # 137109 ; http://n2t.net/addgene:137109 ; RRID:Addgene_137109) -
For your References section:
iFLinkC: an iterative functional linker cloning strategy for the combinatorial assembly and recombination of linker peptides with functional domains. Grawe A, Ranglack J, Weyrich A, Stein V. Nucleic Acids Res. 2020 Jan 11. pii: 5700546. doi: 10.1093/nar/gkz1210. 10.1093/nar/gkz1210 PubMed 31925441