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Addgene

cmv hTid Short H121Q
(Plasmid #13710)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13710 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV bam neo
  • Backbone size w/o insert (bp) 6550
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hTid Short H121Q
  • Alt name
    hTid1
  • Alt name
    DNAJA3
  • Species
    H. sapiens (human); hTid Short H121Q
  • Insert Size (bp)
    2000
  • Mutation
    H121Q
  • GenBank ID
    AF061749
  • Entrez Gene
    DNAJA3 (a.k.a. HCA57, TID1, Tid1-L, Tid1-S, hTID-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (destroyed during cloning)
  • 3′ cloning site BamH1 (destroyed during cloning)
  • 5′ sequencing primer B glob intron-F
  • 3′ sequencing primer B glob-pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    cmv hTid Short H121Q was a gift from Karl Munger (Addgene plasmid # 13710 ; http://n2t.net/addgene:13710 ; RRID:Addgene_13710)

  • For your References section:

    A novel human DnaJ protein, hTid-1, a homolog of the Drosophila tumor suppressor protein Tid56, can interact with the human papillomavirus type 16 E7 oncoprotein. Schilling B, De-Medina T, Syken J, Vidal M, Munger K. Virology. 1998 Jul 20. 247(1):74-85. 10.1006/viro.1998.9220 PubMed 9683573
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