pAP3041
(Plasmid
#137083)
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PurposePlasmid for PCR-generated donor DNA for rapid tagging of proteins with GFP11-3xFlag-tagRFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 137083 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 3168
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Vector typeBacterial Expression ; PCR template for donor DNA
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP11::3xFlag::tagRFP
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Alt namepAP3041-1
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SpeciesH. sapiens (human)
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Insert Size (bp)831
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAP3041 was a gift from Geraldine Seydoux (Addgene plasmid # 137083 ; http://n2t.net/addgene:137083 ; RRID:Addgene_137083) -
For your References section:
Rapid Tagging of Human Proteins with Fluorescent Reporters by Genome Engineering using Double-Stranded DNA Donors. Paix A, Rasoloson D, Folkmann A, Seydoux G. Curr Protoc Mol Biol. 2019 Dec;129(1):e102. doi: 10.1002/cpmb.102. 10.1002/cpmb.102 PubMed 31710422
