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pCC1-4k-BBclone2
(Plasmid #137070)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 137070 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCC1-4k
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    BB0323
  • Species
    Borrelia burgdorferi B31
  • Mutation
    wild-type, full-length (signal-peptide-containing) protein; DNA sequence was optimized for expression in E. coli
  • GenBank ID
    AAC66700.1

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    P13
  • Alt name
    BB0034
  • Species
    Borrelia burgdorferi B31
  • Mutation
    wild-type, full-length (signal-peptide-containing) protein; DNA sequence was optimized for expression in E. coli
  • GenBank ID
    AAC66426.1

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    DipA
  • Alt name
    BB0418
  • Species
    Borrelia burgdorferi B31
  • Mutation
    wild-type, full-length (signal-peptide-containing) protein; DNA sequence was optimized for expression in E. coli
  • GenBank ID
    AAC66790.1

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    Lmp1
  • Alt name
    BB0210 YP_008686569.1
  • Species
    Borrelia burgdorferi B31
  • Mutation
    wild-type, full-length (signal-peptide-containing) protein; DNA sequence was optimized for expression in E. coli
  • GenBank ID
    AAC66595.1

Cloning Information for Gene/Insert 4

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    ordered from GenScript synthesis of the expression-optimized genes

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For use as a PCR template for the expression optimized genes encoding BB0323, P13, DipA, Lmp1. RK2 origin of replication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCC1-4k-BBclone2 was a gift from Debra Hansen (Addgene plasmid # 137070 ; http://n2t.net/addgene:137070 ; RRID:Addgene_137070)

  • For your References section:

    Membrane directed expression in Escherichia coli of BBA57 and other virulence factors from the Lyme disease agent Borrelia burgdorferi. Robertson KE, Truong CD, Craciunescu FM, Chiu PL, Fromme P, Hansen DT. Sci Rep. 2019 Nov 26;9(1):17606. doi: 10.1038/s41598-019-53830-x. 10.1038/s41598-019-53830-x PubMed 31772280
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