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pUC57-BBclone1
(Plasmid #137069)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 137069 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    BB0405
  • Species
    Borrelia burgdorferi B31
  • Mutation
    wild-type, full-length (signal-peptide-containing) protein; DNA sequence was optimized for expression in E. coli
  • GenBank ID
    AAC66795.1
  • Entrez Gene
    BB_0405 (a.k.a. BB_0405)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    BB0406
  • Species
    Borrelia burgdorferi B31
  • Mutation
    wild-type, full-length (signal-peptide-containing) protein; DNA sequence was optimized for expression in E. coli
  • GenBank ID
    AAC66794.1

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    P66
  • Alt name
    BB0603
  • Species
    Borrelia burgdorferi B31
  • Mutation
    wild-type, full-length (signal-peptide-containing) protein; DNA sequence was optimized for expression in E. coli
  • GenBank ID
    AAC66949.1

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    BBA57
  • Species
    Borrelia burgdorferi B31
  • Mutation
    wild-type, full-length (signal-peptide-containing) protein; DNA sequence was optimized for expression in E. coli
  • GenBank ID
    AAC66270.1

Cloning Information for Gene/Insert 4

Gene/Insert 5

  • Gene/Insert name
    HtrA
  • Alt name
    BbHtrA BB0104
  • Species
    Borrelia burgdorferi B31
  • Mutation
    wild-type, full-length (signal-peptide-containing) protein; DNA sequence was optimized for expression in E. coli
  • GenBank ID
    AAC66500.2

Cloning Information for Gene/Insert 5

Gene/Insert 6

  • Gene/Insert name
    RevA
  • Alt name
    Bbm27 Bbp27 AAF07538.1
  • Species
    Borrelia burgdorferi B31
  • Mutation
    wild-type, full-length (signal-peptide-containing) protein; DNA sequence was optimized for expression in E. coli
  • GenBank ID
    AAF07416.1
  • Entrez Gene
    revA (a.k.a. BB_M27)

Cloning Information for Gene/Insert 6

Gene/Insert 7

  • Gene/Insert name
    BB0238
  • Species
    Borrelia burgdorferi B31
  • Mutation
    wild-type, full-length (signal-peptide-containing) protein; DNA sequence was optimized for expression in E. coli
  • GenBank ID
    AAC66635.2

Cloning Information for Gene/Insert 7

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    ordered from GenScript synthesis of the expression-optimized genes

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For use as a PCR template for the expression optimized genes encoding BB0405, BB0406, P66, BBA57, BbHtrA, RevA, BB0238

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC57-BBclone1 was a gift from Debra Hansen (Addgene plasmid # 137069 ; http://n2t.net/addgene:137069 ; RRID:Addgene_137069)

  • For your References section:

    Membrane directed expression in Escherichia coli of BBA57 and other virulence factors from the Lyme disease agent Borrelia burgdorferi. Robertson KE, Truong CD, Craciunescu FM, Chiu PL, Fromme P, Hansen DT. Sci Rep. 2019 Nov 26;9(1):17606. doi: 10.1038/s41598-019-53830-x. 10.1038/s41598-019-53830-x PubMed 31772280
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