pGL2314
(Plasmid
#137054)
-
Purpose(Empty Backbone) loxP (empty) expression cassette: c-terminal 6xHA
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 137054 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneGOI flox pDONR221
-
Vector typeLeishmania floxed gene expression
-
Selectable markersPuromycin
-
Tag
/ Fusion Protein
- 6xHA (C terminal on backbone)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGL2314 was a gift from Jeremy Mottram (Addgene plasmid # 137054 ; http://n2t.net/addgene:137054 ; RRID:Addgene_137054) -
For your References section:
DiCre-Based Inducible Disruption of Leishmania Genes. Duncan SM, Myburgh E, Alves-Ferreira EV, Mottram JC. Methods Mol Biol. 2019;1971:211-224. doi: 10.1007/978-1-4939-9210-2_10. 10.1007/978-1-4939-9210-2_10 PubMed 30980305
