pGL2209
(Plasmid
#137050)
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PurposeDrug Resistance pDONR221 PUR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 137050 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
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Vector typeLeishmania expression gateway vector
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepuromycin N-acetyl-transferase
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL2209 was a gift from Jeremy Mottram (Addgene plasmid # 137050 ; http://n2t.net/addgene:137050 ; RRID:Addgene_137050) -
For your References section:
DiCre-Based Inducible Disruption of Leishmania Genes. Duncan SM, Myburgh E, Alves-Ferreira EV, Mottram JC. Methods Mol Biol. 2019;1971:211-224. doi: 10.1007/978-1-4939-9210-2_10. 10.1007/978-1-4939-9210-2_10 PubMed 30980305