pKnockinDonor-human-p16INK4A-P2A-mCherry
(Plasmid
#137026)
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Purposedonor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4A
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 137026 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescriptSK+
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Backbone manufacturerAddgene
- Backbone size w/o insert (bp) 4486
- Total vector size (bp) 5260
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep16INK4A-knockin-homology arm-P2A-mCherry
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Alt nameP2A-mCherry
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SpeciesP2A-mCherry
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Insert Size (bp)774
- Promoter no promoter
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Tag
/ Fusion Protein
- P2A-mCherry (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13Rev
- 3′ sequencing primer M13For (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKnockinDonor-human-p16INK4A-P2A-mCherry was a gift from Chunliang Li (Addgene plasmid # 137026 ; http://n2t.net/addgene:137026 ; RRID:Addgene_137026) -
For your References section:
A cis-element within the ARF locus mediates repression of p16 (INK4A) expression via long-range chromatin interactions. Zhang Y, Hyle J, Wright S, Shao Y, Zhao X, Zhang H, Li C. Proc Natl Acad Sci U S A. 2019 Dec 9. pii: 1909720116. doi: 10.1073/pnas.1909720116. 10.1073/pnas.1909720116 PubMed 31818950
Map uploaded by the depositor.
