pDD362
(Plasmid
#137023)
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PurposeSapTrap HaloTag-GLO donor
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 137023 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDONR221
- Backbone size w/o insert (bp) 2540
- Total vector size (bp) 3607
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Vector typeWorm Expression, CRISPR ; SapTrap donor for cloning repair templates for C. elegans
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameC. elegans GLO-optimized Halo Tag
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SpeciesC. elegans (nematode), Synthetic
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Insert Size (bp)1047
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Tag
/ Fusion Protein
- C. elegans GLO-optimized Halo Tag
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 Fwd
- 3′ sequencing primer M13 Rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDD362 was a gift from Daniel Dickinson & Bob Goldstein (Addgene plasmid # 137023 ; http://n2t.net/addgene:137023 ; RRID:Addgene_137023)
