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Addgene

pDD289
(Plasmid #137018)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 137018 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19 (modified)
  • Backbone size w/o insert (bp) 3881
  • Total vector size (bp) 10485
  • Modifications to backbone
    Addition of ccdB markers to facilitate homology arm cloning
  • Vector type
    Worm Expression, Cre/Lox, CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Turbo
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP-GLO^SEC^3xFlag + ccdB
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    6605
  • Tags / Fusion Proteins
    • C. elegans GLO-optimized GFP
    • 3xFlag

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDD289 was a gift from Daniel Dickinson & Bob Goldstein (Addgene plasmid # 137018 ; http://n2t.net/addgene:137018 ; RRID:Addgene_137018)
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