pDD288
(Plasmid #137017)

• Purpose: mNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms
• Depositing Labs: Daniel Dickinson, Bob Goldstein
• Publication: Unpublished SapTrap/SEC plasmids (unpublished)

Backbone

• Vector backbone: pUC19 (modified)
• Backbone size w/o insert (bp): 3881
• Total vector size (bp): 10473
• Modifications to backbone: Addition of ccdB markers to facilitate homology arm cloning
• Vector type: Worm Expression, Cre/Lox, CRISPR
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Turbo
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mNG-GLO^SEC^3xFlag + ccdB
• Species: C. elegans (nematode)
• Insert Size (bp): 6593
• Tags / Fusion Proteins:
  • C. elegans GLO-optimized mNG
  • 3xFlag

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13 Forward (tgtaaaacgacggccagt)

Resource Information

• Supplemental Documents:
  • 288 mNG-GLO^SEC^3xFlag ccdB.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry