AAV-SYN_mEGFP-CREB (S133A)
(Plasmid #137010)

• Purpose: AAV backbone, mEGFP-CREB donor for G-CREB with S133A mutation
• Depositing Lab: Ryohei Yasuda
• Publication: Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)31037-2. doi: 10.1016/j.neuron.2019.11.028.

Backbone

• Vector backbone: AAV_pSyn
• Backbone size w/o insert (bp): 4100
• Total vector size (bp): 5500
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CREB1
• Species: R. norvegicus (rat)
• Mutation: Changes Serine 133 into Alanine
• Entrez Gene: Creb1 (a.k.a. Creb)
• Promoter: hSynapsin
• Tag / Fusion Protein:
  • mEGFP (N terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GFP-For

Resource Information

• Supplemental Documents:
  • pAAV-synP-EGFP-CREB (s133a).gbk
• A portion of this plasmid was derived from a plasmid made by: CREB was a gift from Marc Montminy, Addgene #22394

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry