AAV-SYN_mCyRFP2-CREB
(Plasmid
#137008)
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PurposeAAV backbone, mCyRFP2-CREB donor for R-CREB
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 137008 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV_pSyn
- Backbone size w/o insert (bp) 4100
- Total vector size (bp) 5500
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCREB1
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SpeciesR. norvegicus (rat)
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Entrez GeneCreb1 (a.k.a. Creb)
- Promoter hSynapsin
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Tag
/ Fusion Protein
- mCyRFP2 (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer hSyn_For (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byCREB was a gift from Marc Montminy, Addgene #22394
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-SYN_mCyRFP2-CREB was a gift from Ryohei Yasuda (Addgene plasmid # 137008 ; http://n2t.net/addgene:137008 ; RRID:Addgene_137008) -
For your References section:
In Vivo Imaging of the Coupling between Neuronal and CREB Activity in the Mouse Brain. Laviv T, Scholl B, Parra-Bueno P, Foote B, Zhang C, Yan L, Hayano Y, Chu J, Yasuda R. Neuron. 2019 Dec 17. pii: S0896-6273(19)31037-2. doi: 10.1016/j.neuron.2019.11.028. 10.1016/j.neuron.2019.11.028 PubMed 31883788