CMV-mCherry-KIX-mCherry
(Plasmid
#137004)
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PurposemCherry-KIX acceptor for G-CREB
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 137004 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneEGFP-C1
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 5700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKIX domain
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SpeciesR. norvegicus (rat)
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Insert Size (bp)220
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GenBank IDcrebbp
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Entrez GeneCrebbp (a.k.a. CBP, RSTS, RTS)
- Promoter CMV
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Tags
/ Fusion Proteins
- mCherry (N terminal on insert)
- mCherry (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV_For (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byKIX domain was a gift from Oliver Griesbeck
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV-mCherry-KIX-mCherry was a gift from Ryohei Yasuda (Addgene plasmid # 137004 ; http://n2t.net/addgene:137004 ; RRID:Addgene_137004) -
For your References section:
In Vivo Imaging of the Coupling between Neuronal and CREB Activity in the Mouse Brain. Laviv T, Scholl B, Parra-Bueno P, Foote B, Zhang C, Yan L, Hayano Y, Chu J, Yasuda R. Neuron. 2019 Dec 17. pii: S0896-6273(19)31037-2. doi: 10.1016/j.neuron.2019.11.028. 10.1016/j.neuron.2019.11.028 PubMed 31883788