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Addgene

pcDNA3.1-HA-UBA6
(Plasmid #136995)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136995 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UBA6
  • Alt name
    UBE1L2
  • Alt name
    MOP-4, E1-L2
  • Species
    H. sapiens (human)
  • Entrez Gene
    UBA6 (a.k.a. E1-L2, MOP-4, UBE1L2)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Asp-718 (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-HA-UBA6 was a gift from Marcus Groettrup (Addgene plasmid # 136995 ; http://n2t.net/addgene:136995 ; RRID:Addgene_136995)

  • For your References section:

    UBE1L2, a novel E1 enzyme specific for ubiquitin. Pelzer C, Kassner I, Matentzoglu K, Singh RK, Wollscheid HP, Scheffner M, Schmidtke G, Groettrup M. J Biol Chem. 2007 Aug 10;282(32):23010-4. doi: 10.1074/jbc.C700111200. Epub 2007 Jun 19. 10.1074/jbc.C700111200 PubMed 17580310
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