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Addgene

p426*-LbSgH
(Plasmid #136992)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136992 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS426
  • Backbone manufacturer
    Zhao lab
  • Backbone size (bp) 5233
  • Vector type
    Yeast Expression
  • Promoter SNR52
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer gaaagttggtgcgcatg
  • 3′ sequencing primer ggaaacagctatgaccatg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p426*-LbSgH was a gift from Huimin Zhao (Addgene plasmid # 136992 ; http://n2t.net/addgene:136992 ; RRID:Addgene_136992)

  • For your References section:

    Multi-functional genome-wide CRISPR system for high throughput genotype-phenotype mapping. Lian J, Schultz C, Cao M, HamediRad M, Zhao H. Nat Commun. 2019 Dec 19;10(1):5794. doi: 10.1038/s41467-019-13621-4. 10.1038/s41467-019-13621-4 PubMed 31857575
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