pCD/NL-BH*deltavpu/RT-
(Plasmid #136985)

• Purpose: Second-generation lentiviral packaging plasmid lacking reverse transcriptase activity
• Depositing Lab: Jakob Reiser
• Publication: Zhang et al J Virol. 2004 Feb . 78(3):1219-29.

Backbone

• Vector backbone: pcDNA3.1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HIV-1 Gag, Pol (Integrase), Tat, Rev, Vpr, Vif
• Mutation: carries D110E mutation in the reverse transcriptase (RT) coding region

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The pCD/NL-BH*Δvpu RT− plasmid contains sequences (nt 2005 to 5742) derived from pNLNgoMIVR−E−.HSA provided by Dr. Stephen H. Hughes, NCI—Frederick, Frederick, MD
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry