pCD/NL-BH*deltavpu/RT-
(Plasmid
#136985)
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PurposeSecond-generation lentiviral packaging plasmid lacking reverse transcriptase activity
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 136985 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHIV-1 Gag, Pol (Integrase), Tat, Rev, Vpr, Vif
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Mutationcarries D110E mutation in the reverse transcriptase (RT) coding region
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe pCD/NL-BH*Δvpu RT− plasmid contains sequences (nt 2005 to 5742) derived from pNLNgoMIVR−E−.HSA provided by Dr. Stephen H. Hughes, NCI—Frederick, Frederick, MD
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCD/NL-BH*deltavpu/RT- was a gift from Jakob Reiser (Addgene plasmid # 136985 ; http://n2t.net/addgene:136985 ; RRID:Addgene_136985) -
For your References section:
Transduction of bone-marrow-derived mesenchymal stem cells by using lentivirus vectors pseudotyped with modified RD114 envelope glycoproteins. Zhang XY, La Russa VF, Reiser J. J Virol. 2004 Feb . 78(3):1219-29. 10.1128/JVI.78.3.1219-1229.2004 PubMed 14722277
Map uploaded by the depositor.
